Abstract for presentation at 11th International Congress of Human Genetics

Knocking down of Hsp27 radiosensitizes DU145 cancer cells

  • Dr Shahram Teimourian, Immunology, Asthma & Allergy Research Institute, Children Medical Center, Tehran University of Medical Sciences, Iran
  • Dr Razieh Jalla, Laboratory of Biophysics and Molecular Biology, Institute of Biophysics and Biochemistry, University of Tehran, Iran
  • Dr Mostafa Sohrabpour, Laboratory of Biophysics and Molecular Biology, Institute of Biophysics and Biochemistry, University of Tehran, Iran
  • Dr Baharam Goliaei, Laboratory of Biophysics and Molecular Biology, Institute of Biophysics and Biochemistry, University of Tehran, Iran

    • We examined whether human Hsp27is involved in the radiation sensitization induced by gamma radiation in cultured human prostate cancer cells. We transfected DU145 cells with full length Hsp27 antisense cDNA to obtain viable cell lines which expressed reduced Hsp27. Selected individual clones were subjected to Western blot analyses to confirm reduced expression of Hsp27. We showed by colony assays and irradiation at 0,2,4,6 Gy that the cells engineered to express reduced Hsp27 levels had a significantly increased sensitivity to gamma radiation compared with control cells that were transfected with the vector alone. On the other hand there was also a significant difference in viability of cells with reduced Hsp27 levels and control cells 72 hours after gamma irradiation. These results suggest the possible application of antisense Hsp27 cDNA or other methods to reduce Hsp27 expression as a radiation sensitizer in radiation oncology.

    Conference Organiser - ICMS Pty Ltd